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| | So far it has been difficult to clearly separate the emissions of two fluorescence markers in a specimen. With more markers, the problem would grow increasingly complex. Usually, the spectral properties of the available dyes limit the experimental freedom.
For the first time, the unique Emission Fingerprinting technique of the LSM 510 META eliminates the need to define detection bands by filters or other procedures. Now it is possible to separate even widely overlapping emission spectra in three simple steps:
One, have the spectral signature of your specimen automatically recorded in a set of images, the Lambda Stack.
Two, determine reference spectra of the fluorescent markers by the ROI function; you might save them in the LSM Spectra Database for further experiments.
Three, carry out the Linear Unmixing function, which clearly separates the fluorescence signals on the basis of the reference spectra - with speed, ease and certainty.
Another plus of the META detector is the fast electronic switching of detection bands by Metatracking. Like never before, this allows overlapping emission bands to be detected and signal yields to be substantially improved. With these functions, the LSM 510 META lets you see things in a new way, providing clear identification and efficient acquisition of the various fluorescent signals - basic requirements for reliable results even with combinations of dyes that up to now were just impossible to separate.
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