Education in Microscopy and Digital Imaging

Spectral Imaging

Spectral imaging coupled to linear unmixing is becoming an important staple in the microscopist's toolbox, particularly when applied to the elimination of autofluorescence and for FRET investigations. Instruments equipped for spectral imaging are becoming increasingly popular and many confocal microscopes now offer this capability. Widefield fluorescence and brightfield microscopy are also being used more frequently for resolving complex fluorophore and absorbing dye mixtures, a trend that should continue into the future.

Spectral Imaging

Introduction to Spectral Imaging and Linear Unmixing

Spectral imaging combined with linear unmixing is a highly useful technique that can be used in combination with other advanced imaging modalities to untangle fluorescence spectral overlap artifacts in cells and tissues labeled with synthetic fluorophores that would be otherwise difficult to separate.

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Spectral Imaging

FRET Microscopy with Spectral Imaging

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Spectral Imaging

Practical Considerations for Spectral Imaging

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