Interactive Tutorials - Spinning Disk Fundamentals

Reference Library

Spinning Disk Confocal Microscopy

Reference Library

Spinning Disk Confocal Microscopy

Spinning disk confocal microscopy is rapidly emerging as the technique of choice for investigation of dynamics in living cells. Modern commercial instruments and high-performance camera systems are capable of providing high acquisition speeds with acceptable contrast and minimal photobleaching at the low light levels available with this technique. The references listed in this section point to review articles that should provide the starting point for a thorough understanding of spinning disk confocal microscopy.

Live Cell Spinning Disk Microscopy
Advances in Biochemical Engineering/Biotechnology 95: 57-75 (2005)

An excellent review of spinning disk microscopy with emphasis on live-cell imaging. The authors discuss the benefits of spinning disk systems over laser scanning confocal microscopes, including image acquisition rates, photobleaching, multichannel imaging, and optical sectioning. Several examples of biological imaging applications are described.

Gräf, R., Rietdorf, J. and Zimmermann, T.

Performance comparison between the high-speed Yokogawa spinning disk confocal system and single-point scanning confocal systems.
Journal of Microscopy 218: 148-159 (2005)

A comparative investigation of spinning disk and laser scanning confocal microscopy using signal-to-noise and photobleaching as an index of system efficiency. Included are detail discussions of instrument configuration, resolution, photobleaching sensitivity, and saturation effects.


Wang, E., Babbey, C. M. and Dunn, K. W

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